Journal: Pharmaceutics

Article Title: Intra-Domain Cysteines (IDC), a New Strategy for the Development of Original Antibody Fragment–Drug Conjugates (FDCs)

doi: 10.3390/pharmaceutics14081524

Figure Lengend Snippet: Cytotoxicity data for FDCs 4D5.2−MMAF, H0C2.Sx−MMAF (x = 3 to 6) and unconjugated MMAF ( A ) on the HER2-overexpressing cell line SK−BR−3 and ( B ) on the HER2 low expressing cell line MDA−MB−231.

Article Snippet: SK-BR-3 cells were obtained from Cell Lines Service (CLS Eppelheim, Eppelheim, Germany).

Techniques: Expressing

Journal: Biology Open

Article Title: Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development

doi: 10.1242/bio.021063

Figure Lengend Snippet: Postnatal Raldh2cKO mice have decreased neuron numbers in upper cortical layers. (A-J′) Labellings on brain sections from P4 control and Raldh2cKO animals for DAPI (A-B′) and immunolabellings for Cux1 (C-D′), Brn2 (E-F′) or RORβ (I-J′) at rostral (A-J) levels and caudal (A′-J′) levels. (G-H′) Overlay of Cux1 and Brn2 signals. White boxes in A-B′ show the areas selected for the higher magnification pictures of the Cux1, Brn2 and RORβ immunolabellings, and in C-J′ the areas used for cell counts. (K) Quantification of Cux1-positive cells; rostrally: 388.4±17.76 for control and 333.26±12.68 for Raldh2cKO; caudally: 347.6±18.68 for control and 273.73±15.50 for Raldh2cKO. (L) Quantification of Brn2-positive cells; rostrally: 248.26±12.60 for control and 287.2±8.22 for Raldh2cKO; caudally: 221.06±18.91 for control and 243.4±17.53 for Raldh2cKO. (M) Quantification of Cux1-positive/Brn2-negative cells; rostrally: 67.25±5.18 for control and 45.58±1.14 for Raldh2cKO; caudally: 63.66±7.25 for control and 33.83±4.27 for Raldh2cKO. (N) Quantification of RORβ-positive cells; rostrally: 97.33±5.95 for control and 76.8±4.11 for Raldh2cKO; caudally: 84.53±6.73 for control and 59.93±5.71 for Raldh2cKO. Data presented as mean±s.e.m.; n =5 brains; * P <0.05; ns, not significant by two-tailed Student's t -test. Scale bars: 400 μm (A-B′), 100 μm (C-J′).

Article Snippet: After antigen unmasking in citrate buffer (0.01 M, pH 6) during 15 min in a microwave oven, slides were blocked with 5% donkey serum, 0.1% Triton X-100 in phosphate-buffered saline (PBS) and incubated overnight with the following primary antibodies: bromodeoxyuridine (BrdU) (1:500, AbD Serotec #OBT0030G), Ki67 (1:300, Novocastra #NCL-KI67P), phospho-histone H3 (1:500, Upstate #05-806); Pax6 (1:300, Covance #PRB-278P); Tbr2 (1:300, eBioscience #14-4875); βIII-tubulin/Tuj1 (1:200, Covance #MMS-435P-100); Tbr1 (1:100, Abcam #ab31940); Ctip2 (1:500, Abcam #ab18465); Cux1 (1:100, Santa Cruz Biotechnologies #sc13024); cleaved caspase-3 (1:200, R&D Systems #NB100-56113); Brn2 (1:1000 Santa Cruz Biotechnologies #sc6029X), RORβ (1:500, Santa Cruz Biotechnologies #sc21354); Calretinin (1:2000, Swant #7699/4); Raldh2 (1:75, Santa Cruz Biotechnologies #sc22591); nestin (1:100; Developmental Studies Hybridoma Bank #rat-401); laminin (1:500, Sigma #L9393).

Techniques: Control, Two Tailed Test